| Pick
| Team
| Player
| Position
| School (Type)
| Hometown
| Notes
|
| 1 | Pittsburgh Pirates | Bryan Bullington | RHP | Ball State University (Col.) | Indianapolis, IN |
|
| 2 | Tampa Bay Devil Rays | B.J. Upton | SS | Greenbrier Christian Academy High School (HS) | Chesapeake, VA |
|
| 3 | Cincinnati Reds | Chris Gruler | RHP | Liberty High School (HS) | Brentwood, CA |
|
| 4 | Baltimore Orioles | Adam Loewen | LHP | Fraser Valley Christian High School (HS) | Surrey, BC |
|
| 5 | Montreal Expos | Clint Everts | RHP | Cypress Falls High School (HS) | Houston, TX |
|
| 6 | Kansas City Royals | Zack Greinke | RHP | Apopka High School (HS) | Orlando, FL |
|
| 7 | Milwaukee Brewers | Prince Fielder | 1B | Eau Gallie High School (HS) | Melbourne, FL |
|
| 8 | Detroit Tigers | Scott Moore | SS | Cypress High School (HS) | Cypress, CA |
|
| 9 | Colorado Rockies | Jeff Francis | LHP | University of British Columbia (Col.) | Vancouver, BC |
|
| 10 | Texas Rangers | Drew Meyer | SS | University of South Carolina (Col.) | [[]] |
|
| 11 | Florida Marlins | Jeremy Hermida | OF | Wheeler High School (HS) | Marietta, GA |
|
| 12 | Anaheim Angels | Joe Saunders | LHP | Virginia Tech (Col.) | Falls Church, VA |
|
| 13 | San Diego Padres | Khalil Greene | SS | Clemson University (Col.) | Butler, PA |
|
| 14 | Toronto Blue Jays | Russ Adams | SS-2B | University of North Carolina (Col.) | Laurinburg, NC |
|
| 15 | New York Mets | Scott Kazmir | LHP | Cypress Falls High School (HS) | Houston, TX |
|
| 16 | Oakland Athletics | Nick Swisher | 1B-OF | Ohio State University (Col.) | Parkersburg, WV | Compensation from Boston Red Sox for Johnny Damon
|
| 17 | Philadelphia Phillies | Cole Hamels | RHP | Rancho Bernardo High School (HS) | San Diego, CA |
|
| 18 | Chicago White Sox | Royce Ring | LHP | San Diego State University (Col.) | La Mesa, CA |
|
| 19 | Los Angeles Dodgers | James Loney | 1B-LHP | Elkins High School (HS) | Missouri City, TX |
|
| 20 | Minnesota Twins | Denard Span | OF | Tampa Catholic High School (HS) | Tampa, FL |
|
| 21 | Chicago Cubs | Bobby Brownlie | RHP | Rutgers University (Col.) | Edison, NJ |
|
| 22 | Cleveland Indians | Jeremy Guthrie | RHP | Stanford University (Col.) | Roseburg, OR |
|
| 23 | Atlanta Braves | Jeff Francoeur | OF | Parkview High School (HS) | Lilburn, GA |
|
| 24 | Oakland Athletics | Joe Blanton | RHP | University of Kentucky (Col.) | [[]] | Compensation from New York Yankees for Jason Giambi
|
| 25 | San Francisco Giants | Matt Cain | RHP | Houston High School (HS) | Germantown, TN |
|
| 26 | Oakland Athletics | John McCurdy | SS | University of Maryland (Col.) | [[]] |
|
| 27 | Arizona Diamondbacks | Sergio Santos | SS | Mater Dei High School (HS) | Hacienda Heights, CA |
|
| 28 | Seattle Mariners | John Mayberry Jr. | 1B | Rockhurst High School (HS) | Kansas City, MO | Did not sign
|
| 29 | Houston Astros | Derick Grigsby | RHP | Northeast Texas Community College (Col.) | [[]] |
|
| 30 | Oakland Athletics | Ben Fritz | RHP | Fresno State University (Col.) | [[]] | Compensation from St. Louis Cardinals for Jason Isringhausen
|
